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Silicon inhibition effects on the polymerase chain reaction: A real‐time detection approach

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Zeitschriftentitel: Journal of Biomedical Materials Research Part A
Personen und Körperschaften: Wang, Wei, Wang, Hai‐Bin, Li, Zhi‐Xin, Guo, Zeng‐Yuan
In: Journal of Biomedical Materials Research Part A, 77A, 2006, 1, S. 28-34
Medientyp: E-Article
Sprache: Englisch
veröffentlicht:
Wiley
Schlagwörter:
author_facet Wang, Wei
Wang, Hai‐Bin
Li, Zhi‐Xin
Guo, Zeng‐Yuan
Wang, Wei
Wang, Hai‐Bin
Li, Zhi‐Xin
Guo, Zeng‐Yuan
author Wang, Wei
Wang, Hai‐Bin
Li, Zhi‐Xin
Guo, Zeng‐Yuan
spellingShingle Wang, Wei
Wang, Hai‐Bin
Li, Zhi‐Xin
Guo, Zeng‐Yuan
Journal of Biomedical Materials Research Part A
Silicon inhibition effects on the polymerase chain reaction: A real‐time detection approach
Metals and Alloys
Biomedical Engineering
Biomaterials
Ceramics and Composites
author_sort wang, wei
spelling Wang, Wei Wang, Hai‐Bin Li, Zhi‐Xin Guo, Zeng‐Yuan 1549-3296 1552-4965 Wiley Metals and Alloys Biomedical Engineering Biomaterials Ceramics and Composites http://dx.doi.org/10.1002/jbm.a.30627 <jats:title>Abstract</jats:title><jats:p>In the miniaturization of biochemical analysis systems, biocompatibility of the microfabricated material is a key feature to be considered. A clear insight into interactions between biological reagents and microchip materials will help to build more robust functional bio‐microelectromechanical systems (BioMEMS). In the present work, a real‐time polymerase chain reaction (PCR) assay was used to study the inhibition effects of silicon and native silicon oxide particles on Hepatitis B Virus (HBV) DNA PCR amplification. Silicon nanoparticles with different surface oxides were added into the PCR mixture to activate possible interactions between the silicon‐related materials and the PCR reagents. Ratios of silicon nanoparticle surface area to PCR mixture volume (surface to volume ratio) varied from 4.7 to 235.5 mm<jats:sup>2</jats:sup>/μL. Using high speed centrifugation, the nanoparticles were pelleted to tube inner surfaces. Supernatant extracts were then used in subsequent PCR experiments. To test whether silicon materials participated in amplifications directly, in some cases, entire PCR mixture containing silicon nanoparticles were used in amplification. Fluorescence histories of PCR amplifications indicated that with the increase in surface to volume ratio, amplification efficiency decreased considerably, and within the studied ranges, the higher the particle surface oxidation, the stronger the silicon inhibition effects on PCR. Adsorption of Taq polymerase (not nucleic acid) on the silicon‐related material surface was the primary cause of the inhibition phenomena and silicon did not participate in the amplification process directly. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res, 2006</jats:p> Silicon inhibition effects on the polymerase chain reaction: A real‐time detection approach Journal of Biomedical Materials Research Part A
doi_str_mv 10.1002/jbm.a.30627
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series Journal of Biomedical Materials Research Part A
source_id 49
title Silicon inhibition effects on the polymerase chain reaction: A real‐time detection approach
title_unstemmed Silicon inhibition effects on the polymerase chain reaction: A real‐time detection approach
title_full Silicon inhibition effects on the polymerase chain reaction: A real‐time detection approach
title_fullStr Silicon inhibition effects on the polymerase chain reaction: A real‐time detection approach
title_full_unstemmed Silicon inhibition effects on the polymerase chain reaction: A real‐time detection approach
title_short Silicon inhibition effects on the polymerase chain reaction: A real‐time detection approach
title_sort silicon inhibition effects on the polymerase chain reaction: a real‐time detection approach
topic Metals and Alloys
Biomedical Engineering
Biomaterials
Ceramics and Composites
url http://dx.doi.org/10.1002/jbm.a.30627
publishDate 2006
physical 28-34
description <jats:title>Abstract</jats:title><jats:p>In the miniaturization of biochemical analysis systems, biocompatibility of the microfabricated material is a key feature to be considered. A clear insight into interactions between biological reagents and microchip materials will help to build more robust functional bio‐microelectromechanical systems (BioMEMS). In the present work, a real‐time polymerase chain reaction (PCR) assay was used to study the inhibition effects of silicon and native silicon oxide particles on Hepatitis B Virus (HBV) DNA PCR amplification. Silicon nanoparticles with different surface oxides were added into the PCR mixture to activate possible interactions between the silicon‐related materials and the PCR reagents. Ratios of silicon nanoparticle surface area to PCR mixture volume (surface to volume ratio) varied from 4.7 to 235.5 mm<jats:sup>2</jats:sup>/μL. Using high speed centrifugation, the nanoparticles were pelleted to tube inner surfaces. Supernatant extracts were then used in subsequent PCR experiments. To test whether silicon materials participated in amplifications directly, in some cases, entire PCR mixture containing silicon nanoparticles were used in amplification. Fluorescence histories of PCR amplifications indicated that with the increase in surface to volume ratio, amplification efficiency decreased considerably, and within the studied ranges, the higher the particle surface oxidation, the stronger the silicon inhibition effects on PCR. Adsorption of Taq polymerase (not nucleic acid) on the silicon‐related material surface was the primary cause of the inhibition phenomena and silicon did not participate in the amplification process directly. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res, 2006</jats:p>
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author Wang, Wei, Wang, Hai‐Bin, Li, Zhi‐Xin, Guo, Zeng‐Yuan
author_facet Wang, Wei, Wang, Hai‐Bin, Li, Zhi‐Xin, Guo, Zeng‐Yuan, Wang, Wei, Wang, Hai‐Bin, Li, Zhi‐Xin, Guo, Zeng‐Yuan
author_sort wang, wei
container_issue 1
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container_title Journal of Biomedical Materials Research Part A
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description <jats:title>Abstract</jats:title><jats:p>In the miniaturization of biochemical analysis systems, biocompatibility of the microfabricated material is a key feature to be considered. A clear insight into interactions between biological reagents and microchip materials will help to build more robust functional bio‐microelectromechanical systems (BioMEMS). In the present work, a real‐time polymerase chain reaction (PCR) assay was used to study the inhibition effects of silicon and native silicon oxide particles on Hepatitis B Virus (HBV) DNA PCR amplification. Silicon nanoparticles with different surface oxides were added into the PCR mixture to activate possible interactions between the silicon‐related materials and the PCR reagents. Ratios of silicon nanoparticle surface area to PCR mixture volume (surface to volume ratio) varied from 4.7 to 235.5 mm<jats:sup>2</jats:sup>/μL. Using high speed centrifugation, the nanoparticles were pelleted to tube inner surfaces. Supernatant extracts were then used in subsequent PCR experiments. To test whether silicon materials participated in amplifications directly, in some cases, entire PCR mixture containing silicon nanoparticles were used in amplification. Fluorescence histories of PCR amplifications indicated that with the increase in surface to volume ratio, amplification efficiency decreased considerably, and within the studied ranges, the higher the particle surface oxidation, the stronger the silicon inhibition effects on PCR. Adsorption of Taq polymerase (not nucleic acid) on the silicon‐related material surface was the primary cause of the inhibition phenomena and silicon did not participate in the amplification process directly. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res, 2006</jats:p>
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spelling Wang, Wei Wang, Hai‐Bin Li, Zhi‐Xin Guo, Zeng‐Yuan 1549-3296 1552-4965 Wiley Metals and Alloys Biomedical Engineering Biomaterials Ceramics and Composites http://dx.doi.org/10.1002/jbm.a.30627 <jats:title>Abstract</jats:title><jats:p>In the miniaturization of biochemical analysis systems, biocompatibility of the microfabricated material is a key feature to be considered. A clear insight into interactions between biological reagents and microchip materials will help to build more robust functional bio‐microelectromechanical systems (BioMEMS). In the present work, a real‐time polymerase chain reaction (PCR) assay was used to study the inhibition effects of silicon and native silicon oxide particles on Hepatitis B Virus (HBV) DNA PCR amplification. Silicon nanoparticles with different surface oxides were added into the PCR mixture to activate possible interactions between the silicon‐related materials and the PCR reagents. Ratios of silicon nanoparticle surface area to PCR mixture volume (surface to volume ratio) varied from 4.7 to 235.5 mm<jats:sup>2</jats:sup>/μL. Using high speed centrifugation, the nanoparticles were pelleted to tube inner surfaces. Supernatant extracts were then used in subsequent PCR experiments. To test whether silicon materials participated in amplifications directly, in some cases, entire PCR mixture containing silicon nanoparticles were used in amplification. Fluorescence histories of PCR amplifications indicated that with the increase in surface to volume ratio, amplification efficiency decreased considerably, and within the studied ranges, the higher the particle surface oxidation, the stronger the silicon inhibition effects on PCR. Adsorption of Taq polymerase (not nucleic acid) on the silicon‐related material surface was the primary cause of the inhibition phenomena and silicon did not participate in the amplification process directly. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res, 2006</jats:p> Silicon inhibition effects on the polymerase chain reaction: A real‐time detection approach Journal of Biomedical Materials Research Part A
spellingShingle Wang, Wei, Wang, Hai‐Bin, Li, Zhi‐Xin, Guo, Zeng‐Yuan, Journal of Biomedical Materials Research Part A, Silicon inhibition effects on the polymerase chain reaction: A real‐time detection approach, Metals and Alloys, Biomedical Engineering, Biomaterials, Ceramics and Composites
title Silicon inhibition effects on the polymerase chain reaction: A real‐time detection approach
title_full Silicon inhibition effects on the polymerase chain reaction: A real‐time detection approach
title_fullStr Silicon inhibition effects on the polymerase chain reaction: A real‐time detection approach
title_full_unstemmed Silicon inhibition effects on the polymerase chain reaction: A real‐time detection approach
title_short Silicon inhibition effects on the polymerase chain reaction: A real‐time detection approach
title_sort silicon inhibition effects on the polymerase chain reaction: a real‐time detection approach
title_unstemmed Silicon inhibition effects on the polymerase chain reaction: A real‐time detection approach
topic Metals and Alloys, Biomedical Engineering, Biomaterials, Ceramics and Composites
url http://dx.doi.org/10.1002/jbm.a.30627