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Regulation of cell arrangement using a novel composite micropattern
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| Zeitschriftentitel: | Journal of Biomedical Materials Research Part A |
|---|---|
| Personen und Körperschaften: | , , , , , , , , , |
| In: | Journal of Biomedical Materials Research Part A, 105, 2017, 11, S. 3093-3101 |
| Medientyp: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
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| Schlagwörter: |
| author_facet |
Liu, Xiaoyi Liu, Yaoping Zhao, Feng Hun, Tingting Li, Shan Wang, Yuguang Sun, Weijie Wang, Wei Sun, Yan Fan, Yubo Liu, Xiaoyi Liu, Yaoping Zhao, Feng Hun, Tingting Li, Shan Wang, Yuguang Sun, Weijie Wang, Wei Sun, Yan Fan, Yubo |
|---|---|
| author |
Liu, Xiaoyi Liu, Yaoping Zhao, Feng Hun, Tingting Li, Shan Wang, Yuguang Sun, Weijie Wang, Wei Sun, Yan Fan, Yubo |
| spellingShingle |
Liu, Xiaoyi Liu, Yaoping Zhao, Feng Hun, Tingting Li, Shan Wang, Yuguang Sun, Weijie Wang, Wei Sun, Yan Fan, Yubo Journal of Biomedical Materials Research Part A Regulation of cell arrangement using a novel composite micropattern Metals and Alloys Biomedical Engineering Biomaterials Ceramics and Composites |
| author_sort |
liu, xiaoyi |
| spelling |
Liu, Xiaoyi Liu, Yaoping Zhao, Feng Hun, Tingting Li, Shan Wang, Yuguang Sun, Weijie Wang, Wei Sun, Yan Fan, Yubo 1549-3296 1552-4965 Wiley Metals and Alloys Biomedical Engineering Biomaterials Ceramics and Composites http://dx.doi.org/10.1002/jbm.a.36157 <jats:title>Abstract</jats:title><jats:p>Micropatterning technique has been used to control single cell geometry in many researches, however, this is no report that it is used to control multicelluar geometry, which not only control single cell geometry but also organize those cells by a certain pattern. In this work, a composite protein micropattern is developed to control both cell shape and cell location simultaneously. The composite micropattern consists of a central circle 15 μm in diameter for single‐cell capture, surrounded by small, square arrays (3 μm × 3 μm) for cell spreading. This is surrounded by a border 2 μm wide for restricting cell edges. The composite pattern results in two‐cell and three‐cell capture efficiencies of 32.1% ± 1.94% and 24.2% ± 2.89%, respectively, representing an 8.52% and 9.58% increase, respectively, over rates of original patterns. Fluorescent imaging of cytoskeleton alignment demonstrates that actin is gradually aligned parallel to the direction of the entire pattern arrangement, rather than to that of a single pattern. This indicates that cell arrangement is also an important factor in determining cell physiology. This composite micropattern could be a potential method to precisely control multi‐cells for cell junctions, cell interactions, cell signal transduction, and eventually for tissue rebuilding study. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 3093–3101, 2017.</jats:p> Regulation of cell arrangement using a novel composite micropattern Journal of Biomedical Materials Research Part A |
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Regulation of cell arrangement using a novel composite micropattern |
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Regulation of cell arrangement using a novel composite micropattern |
| title_full |
Regulation of cell arrangement using a novel composite micropattern |
| title_fullStr |
Regulation of cell arrangement using a novel composite micropattern |
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Regulation of cell arrangement using a novel composite micropattern |
| title_short |
Regulation of cell arrangement using a novel composite micropattern |
| title_sort |
regulation of cell arrangement using a novel composite micropattern |
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Metals and Alloys Biomedical Engineering Biomaterials Ceramics and Composites |
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http://dx.doi.org/10.1002/jbm.a.36157 |
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2017 |
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3093-3101 |
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<jats:title>Abstract</jats:title><jats:p>Micropatterning technique has been used to control single cell geometry in many researches, however, this is no report that it is used to control multicelluar geometry, which not only control single cell geometry but also organize those cells by a certain pattern. In this work, a composite protein micropattern is developed to control both cell shape and cell location simultaneously. The composite micropattern consists of a central circle 15 μm in diameter for single‐cell capture, surrounded by small, square arrays (3 μm × 3 μm) for cell spreading. This is surrounded by a border 2 μm wide for restricting cell edges. The composite pattern results in two‐cell and three‐cell capture efficiencies of 32.1% ± 1.94% and 24.2% ± 2.89%, respectively, representing an 8.52% and 9.58% increase, respectively, over rates of original patterns. Fluorescent imaging of cytoskeleton alignment demonstrates that actin is gradually aligned parallel to the direction of the entire pattern arrangement, rather than to that of a single pattern. This indicates that cell arrangement is also an important factor in determining cell physiology. This composite micropattern could be a potential method to precisely control multi‐cells for cell junctions, cell interactions, cell signal transduction, and eventually for tissue rebuilding study. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 3093–3101, 2017.</jats:p> |
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| author | Liu, Xiaoyi, Liu, Yaoping, Zhao, Feng, Hun, Tingting, Li, Shan, Wang, Yuguang, Sun, Weijie, Wang, Wei, Sun, Yan, Fan, Yubo |
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| description | <jats:title>Abstract</jats:title><jats:p>Micropatterning technique has been used to control single cell geometry in many researches, however, this is no report that it is used to control multicelluar geometry, which not only control single cell geometry but also organize those cells by a certain pattern. In this work, a composite protein micropattern is developed to control both cell shape and cell location simultaneously. The composite micropattern consists of a central circle 15 μm in diameter for single‐cell capture, surrounded by small, square arrays (3 μm × 3 μm) for cell spreading. This is surrounded by a border 2 μm wide for restricting cell edges. The composite pattern results in two‐cell and three‐cell capture efficiencies of 32.1% ± 1.94% and 24.2% ± 2.89%, respectively, representing an 8.52% and 9.58% increase, respectively, over rates of original patterns. Fluorescent imaging of cytoskeleton alignment demonstrates that actin is gradually aligned parallel to the direction of the entire pattern arrangement, rather than to that of a single pattern. This indicates that cell arrangement is also an important factor in determining cell physiology. This composite micropattern could be a potential method to precisely control multi‐cells for cell junctions, cell interactions, cell signal transduction, and eventually for tissue rebuilding study. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 3093–3101, 2017.</jats:p> |
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| spelling | Liu, Xiaoyi Liu, Yaoping Zhao, Feng Hun, Tingting Li, Shan Wang, Yuguang Sun, Weijie Wang, Wei Sun, Yan Fan, Yubo 1549-3296 1552-4965 Wiley Metals and Alloys Biomedical Engineering Biomaterials Ceramics and Composites http://dx.doi.org/10.1002/jbm.a.36157 <jats:title>Abstract</jats:title><jats:p>Micropatterning technique has been used to control single cell geometry in many researches, however, this is no report that it is used to control multicelluar geometry, which not only control single cell geometry but also organize those cells by a certain pattern. In this work, a composite protein micropattern is developed to control both cell shape and cell location simultaneously. The composite micropattern consists of a central circle 15 μm in diameter for single‐cell capture, surrounded by small, square arrays (3 μm × 3 μm) for cell spreading. This is surrounded by a border 2 μm wide for restricting cell edges. The composite pattern results in two‐cell and three‐cell capture efficiencies of 32.1% ± 1.94% and 24.2% ± 2.89%, respectively, representing an 8.52% and 9.58% increase, respectively, over rates of original patterns. Fluorescent imaging of cytoskeleton alignment demonstrates that actin is gradually aligned parallel to the direction of the entire pattern arrangement, rather than to that of a single pattern. This indicates that cell arrangement is also an important factor in determining cell physiology. This composite micropattern could be a potential method to precisely control multi‐cells for cell junctions, cell interactions, cell signal transduction, and eventually for tissue rebuilding study. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 3093–3101, 2017.</jats:p> Regulation of cell arrangement using a novel composite micropattern Journal of Biomedical Materials Research Part A |
| spellingShingle | Liu, Xiaoyi, Liu, Yaoping, Zhao, Feng, Hun, Tingting, Li, Shan, Wang, Yuguang, Sun, Weijie, Wang, Wei, Sun, Yan, Fan, Yubo, Journal of Biomedical Materials Research Part A, Regulation of cell arrangement using a novel composite micropattern, Metals and Alloys, Biomedical Engineering, Biomaterials, Ceramics and Composites |
| title | Regulation of cell arrangement using a novel composite micropattern |
| title_full | Regulation of cell arrangement using a novel composite micropattern |
| title_fullStr | Regulation of cell arrangement using a novel composite micropattern |
| title_full_unstemmed | Regulation of cell arrangement using a novel composite micropattern |
| title_short | Regulation of cell arrangement using a novel composite micropattern |
| title_sort | regulation of cell arrangement using a novel composite micropattern |
| title_unstemmed | Regulation of cell arrangement using a novel composite micropattern |
| topic | Metals and Alloys, Biomedical Engineering, Biomaterials, Ceramics and Composites |
| url | http://dx.doi.org/10.1002/jbm.a.36157 |