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Buchumschlag von Localisation of Two Bioactive Labdane Diterpenoids in the Peltate Glandular Trichomes of Leonurus japonicus by Laser Microdissection Coupled with UPLC‐MS/MS
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Localisation of Two Bioactive Labdane Diterpenoids in the Peltate Glandular Trichomes of Leonurus japonicus by Laser Microdissection Coupled with UPLC‐MS/MS

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Zeitschriftentitel: Phytochemical Analysis
Personen und Körperschaften: Xiao, Chao‐Jiang, Liu, Yan‐Chun, Luo, Shi‐Hong, Hua, Juan, Liu, Yan, Li, Sheng‐Hong
In: Phytochemical Analysis, 28, 2017, 5, S. 404-409
Medientyp: E-Article
Sprache: Englisch
veröffentlicht:
Wiley
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Zusammenfassung: <jats:title>Abstract</jats:title><jats:sec><jats:title>Introduction</jats:title><jats:p>Glandular trichomes of plants are biochemical factories that could produce, store and secrete copious pharmaceutically important natural products. The Labiatae plant <jats:styled-content style="fixed-case"><jats:italic>Leonurus japonicus</jats:italic></jats:styled-content> is an important traditional Chinese medicine used to treat gynecological diseases, and has abundant peltate glandular trichomes (PGTs), in which the secondary metabolites accumulated are still unknown.</jats:p></jats:sec><jats:sec><jats:title>Objective</jats:title><jats:p>To study the secondary metabolites specifically accumulated in the PGTs of <jats:styled-content style="fixed-case"><jats:italic>L. japonicus</jats:italic></jats:styled-content> and their biological activities, and provide a new way to pinpoint bioactive natural products from plants.</jats:p></jats:sec><jats:sec><jats:title>Methodology</jats:title><jats:p>Morphology of the trichomes on <jats:styled-content style="fixed-case"><jats:italic>L. japonicus</jats:italic></jats:styled-content> were observed under a scanning electron microscope. The PGTs of <jats:styled-content style="fixed-case"><jats:italic>L. japonicus</jats:italic></jats:styled-content> were precisely collected using laser microdissection (LMD) and analysed for their secondary metabolites with ultra performance liquid chromatography‐tandem mass spectrometry (UPLC‐MS/MS). Targeted compounds were isolated with classical phytochemical methods, and their structures were elucidated by spectroscopic analysis. Biological activities were evaluated by <jats:italic>in vitro</jats:italic> assays.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>Two labdane diterpenoids, leoheterin (1) and galeopsin (2), were localised in the PGTs of <jats:styled-content style="fixed-case"><jats:italic>L. japonicus</jats:italic></jats:styled-content>. Antithrombotic activity of 1 in anti‐platelet aggregation assay induced by arachidonic acid was observed. Both compounds showed potential anti‐inflammatory activity by inhibiting proinflammatory cytokine TNF‐α. In addition, anti‐proliferative effect of both compounds on several cancer cell lines was also detected.</jats:p></jats:sec><jats:sec><jats:title>Conclusion</jats:title><jats:p>Two bioactive labdane diterpenoids were localised in the PGTs of <jats:styled-content style="fixed-case"><jats:italic>L. japonicus</jats:italic></jats:styled-content>. The findings suggested that it might be an efficient approach to explore bioactive natural products from the glandular trichomes of medicinal plants with LMD‐UPLC/MS/MS. Copyright © 2017 John Wiley &amp; Sons, Ltd.</jats:p></jats:sec>
Umfang: 404-409
ISSN: 0958-0344
1099-1565
DOI: 10.1002/pca.2687