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Bone morphogenetic protein-5 and early endothelial outgrowth cells (eEOCs) in acute ischemic kidney...

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Journal Title: American Journal of Physiology-Renal Physiology
Authors and Corporations: Patschan, Daniel, Schwarze, Katrin, Lange, Andrea, Meise, Nyree, Henze, Elvira, Becker, Jan Ulrich, Patschan, Susann, Müller, Gerhard A.
In: American Journal of Physiology-Renal Physiology, 305, 2013, 3, p. F314-F322
Media Type: E-Article
Language: English
published:
American Physiological Society
Subjects:
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rft.atitle Bone morphogenetic protein-5 and early endothelial outgrowth cells (eEOCs) in acute ischemic kidney injury (AKI) and 5/6-chronic kidney disease
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rft.jtitle American Journal of Physiology-Renal Physiology
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rft.date 2013-08-01
x.date 2013-08-01T00:00:00Z
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abstract <jats:p> Early endothelial outgrowth cells (eEOCs) reproducibly have been shown to act protectively in acute ischemic kidney injury (AKI) and chronic kidney injury. Bone morphogenetic protein-5 (BMP-5) acted antifibrotically in human hypertensive nephropathy. The aim of the curent study was to analyze effects of BMP-5 treatment in an eEOC-based therapy of murine AKI and 5/6-nephrectomy. Male C57/Bl6N mice were either subjected to unilateral renal artery clamping postuninephrectomy or to 5/6-nephrectomy. Untreated or BMP-5-pretreated murine eEOCs were injected into recipient animals at the time of reperfusion (AKI) or at 2 and 5 days after 5/6-nephrectomy. Analysis of renal function and morphology was performed at 48 h and at 6 wk (AKI) or at 8 wk (5/6 model). Cellular consequences of eEOC treatment were evaluated using different in vitro assays. AKI was mitigated significantly by injecting BMP-5-pretreated eEOCs. Renal function was improved at 48 h and at 6 wk after cell therapy. In 5/6-nephrectomy, the cells failed to protect renal function, but proteinuria was reduced after administering untreated eEOCs. BMP-5 pretreatment resulted in aggravated proteinuria and renal fibrosis. In 5/6-nephrectomized animals, percentages of anti-smooth muscle actin+/CD31+ cells increased, indicating endothelial-mesenchymal transition (EnMT). In vitro analysis revealed increased cell migration and reduced cell apoptosis/necrosis. Paracrinic activity remained unaffected. BMP-5 acts as a potent eEOC agonist in murine AKI in the short and mid to long term. Cell effects in 5/6-nephrectomy are heterogenous, but untreated cells act antiproteinurically and antifibrotically without any impact on EnMT. </jats:p>
authors Array ( [rft.aulast] => Patschan [rft.aufirst] => Daniel )
Array ( [rft.aulast] => Schwarze [rft.aufirst] => Katrin )
Array ( [rft.aulast] => Lange [rft.aufirst] => Andrea )
Array ( [rft.aulast] => Meise [rft.aufirst] => Nyree )
Array ( [rft.aulast] => Henze [rft.aufirst] => Elvira )
Array ( [rft.aulast] => Becker [rft.aufirst] => Jan Ulrich )
Array ( [rft.aulast] => Patschan [rft.aufirst] => Susann )
Array ( [rft.aulast] => Müller [rft.aufirst] => Gerhard A. )
doi 10.1152/ajprenal.00677.2012
languages eng
url http://dx.doi.org/10.1152/ajprenal.00677.2012
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Physiology
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author Patschan, Daniel, Schwarze, Katrin, Lange, Andrea, Meise, Nyree, Henze, Elvira, Becker, Jan Ulrich, Patschan, Susann, Müller, Gerhard A.
author_facet Patschan, Daniel, Schwarze, Katrin, Lange, Andrea, Meise, Nyree, Henze, Elvira, Becker, Jan Ulrich, Patschan, Susann, Müller, Gerhard A., Patschan, Daniel, Schwarze, Katrin, Lange, Andrea, Meise, Nyree, Henze, Elvira, Becker, Jan Ulrich, Patschan, Susann, Müller, Gerhard A.
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container_title American Journal of Physiology-Renal Physiology
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description <jats:p> Early endothelial outgrowth cells (eEOCs) reproducibly have been shown to act protectively in acute ischemic kidney injury (AKI) and chronic kidney injury. Bone morphogenetic protein-5 (BMP-5) acted antifibrotically in human hypertensive nephropathy. The aim of the curent study was to analyze effects of BMP-5 treatment in an eEOC-based therapy of murine AKI and 5/6-nephrectomy. Male C57/Bl6N mice were either subjected to unilateral renal artery clamping postuninephrectomy or to 5/6-nephrectomy. Untreated or BMP-5-pretreated murine eEOCs were injected into recipient animals at the time of reperfusion (AKI) or at 2 and 5 days after 5/6-nephrectomy. Analysis of renal function and morphology was performed at 48 h and at 6 wk (AKI) or at 8 wk (5/6 model). Cellular consequences of eEOC treatment were evaluated using different in vitro assays. AKI was mitigated significantly by injecting BMP-5-pretreated eEOCs. Renal function was improved at 48 h and at 6 wk after cell therapy. In 5/6-nephrectomy, the cells failed to protect renal function, but proteinuria was reduced after administering untreated eEOCs. BMP-5 pretreatment resulted in aggravated proteinuria and renal fibrosis. In 5/6-nephrectomized animals, percentages of anti-smooth muscle actin+/CD31+ cells increased, indicating endothelial-mesenchymal transition (EnMT). In vitro analysis revealed increased cell migration and reduced cell apoptosis/necrosis. Paracrinic activity remained unaffected. BMP-5 acts as a potent eEOC agonist in murine AKI in the short and mid to long term. Cell effects in 5/6-nephrectomy are heterogenous, but untreated cells act antiproteinurically and antifibrotically without any impact on EnMT. </jats:p>
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spelling Patschan, Daniel Schwarze, Katrin Lange, Andrea Meise, Nyree Henze, Elvira Becker, Jan Ulrich Patschan, Susann Müller, Gerhard A. 1931-857X 1522-1466 American Physiological Society Urology Physiology http://dx.doi.org/10.1152/ajprenal.00677.2012 <jats:p> Early endothelial outgrowth cells (eEOCs) reproducibly have been shown to act protectively in acute ischemic kidney injury (AKI) and chronic kidney injury. Bone morphogenetic protein-5 (BMP-5) acted antifibrotically in human hypertensive nephropathy. The aim of the curent study was to analyze effects of BMP-5 treatment in an eEOC-based therapy of murine AKI and 5/6-nephrectomy. Male C57/Bl6N mice were either subjected to unilateral renal artery clamping postuninephrectomy or to 5/6-nephrectomy. Untreated or BMP-5-pretreated murine eEOCs were injected into recipient animals at the time of reperfusion (AKI) or at 2 and 5 days after 5/6-nephrectomy. Analysis of renal function and morphology was performed at 48 h and at 6 wk (AKI) or at 8 wk (5/6 model). Cellular consequences of eEOC treatment were evaluated using different in vitro assays. AKI was mitigated significantly by injecting BMP-5-pretreated eEOCs. Renal function was improved at 48 h and at 6 wk after cell therapy. In 5/6-nephrectomy, the cells failed to protect renal function, but proteinuria was reduced after administering untreated eEOCs. BMP-5 pretreatment resulted in aggravated proteinuria and renal fibrosis. In 5/6-nephrectomized animals, percentages of anti-smooth muscle actin+/CD31+ cells increased, indicating endothelial-mesenchymal transition (EnMT). In vitro analysis revealed increased cell migration and reduced cell apoptosis/necrosis. Paracrinic activity remained unaffected. BMP-5 acts as a potent eEOC agonist in murine AKI in the short and mid to long term. Cell effects in 5/6-nephrectomy are heterogenous, but untreated cells act antiproteinurically and antifibrotically without any impact on EnMT. </jats:p> Bone morphogenetic protein-5 and early endothelial outgrowth cells (eEOCs) in acute ischemic kidney injury (AKI) and 5/6-chronic kidney disease American Journal of Physiology-Renal Physiology
spellingShingle Patschan, Daniel, Schwarze, Katrin, Lange, Andrea, Meise, Nyree, Henze, Elvira, Becker, Jan Ulrich, Patschan, Susann, Müller, Gerhard A., American Journal of Physiology-Renal Physiology, Bone morphogenetic protein-5 and early endothelial outgrowth cells (eEOCs) in acute ischemic kidney injury (AKI) and 5/6-chronic kidney disease, Urology, Physiology
title Bone morphogenetic protein-5 and early endothelial outgrowth cells (eEOCs) in acute ischemic kidney injury (AKI) and 5/6-chronic kidney disease
title_full Bone morphogenetic protein-5 and early endothelial outgrowth cells (eEOCs) in acute ischemic kidney injury (AKI) and 5/6-chronic kidney disease
title_fullStr Bone morphogenetic protein-5 and early endothelial outgrowth cells (eEOCs) in acute ischemic kidney injury (AKI) and 5/6-chronic kidney disease
title_full_unstemmed Bone morphogenetic protein-5 and early endothelial outgrowth cells (eEOCs) in acute ischemic kidney injury (AKI) and 5/6-chronic kidney disease
title_short Bone morphogenetic protein-5 and early endothelial outgrowth cells (eEOCs) in acute ischemic kidney injury (AKI) and 5/6-chronic kidney disease
title_sort bone morphogenetic protein-5 and early endothelial outgrowth cells (eeocs) in acute ischemic kidney injury (aki) and 5/6-chronic kidney disease
topic Urology, Physiology
url http://dx.doi.org/10.1152/ajprenal.00677.2012