%0 Electronic Article %A Mitra, Alok K. %I International Union of Crystallography (IUCr) %D 2019 %D 2019 %G Undetermined %@ 2053-230X %~ Katalog UB TU-Chemnitz %T Visualization of biological macromolecules at near-atomic resolution: cryo-electron microscopy comes of age %V 75 %J Acta Crystallographica Section F Structural Biology Communications %V 75 %N 1 %P 3-11 %U http://dx.doi.org/10.1107/s2053230x18015133 %X Structural biology is going through a revolution as a result of transformational advances in the field of cryo-electron microscopy (cryo-EM) driven by the development of direct electron detectors and ultrastable electron microscopes. High-resolution cryo-EM images of isolated biomolecules (single particles) suspended in a thin layer of vitrified buffer are subjected to powerful image-processing algorithms, enabling near-atomic resolution structures to be determined in unprecedented numbers. Prior to these advances, electron crystallography of two-dimensional crystals and helical assemblies of proteins had established the feasibility of atomic resolution structure determination using cryo-EM. Atomic resolution single-particle analysis, without the need for crystals, now promises to resolve problems in structural biology that were intractable just a few years ago. %Z https://katalog.bibliothek.tu-chemnitz.de/Record/ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTEwNy9zMjA1MzIzMHgxODAxNTEzMw %U https://katalog.bibliothek.tu-chemnitz.de/Record/ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTEwNy9zMjA1MzIzMHgxODAxNTEzMw