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A splicing mutation in PHKG1 decreased its expression in skeletal muscle and caused PSE meat in Duroc × Luchuan crossbred pigs
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Zeitschriftentitel: | Animal Genetics |
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Personen und Körperschaften: | , , , , , , , , , , |
In: | Animal Genetics, 50, 2019, 4, S. 395-398 |
Medientyp: | E-Article |
Sprache: | Englisch |
veröffentlicht: |
Wiley
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Schlagwörter: |
Zusammenfassung: | <jats:title>Summary</jats:title><jats:p>In recent years, Luchuan pigs in southern China have been used to produce high‐quality meat by crossbreeding them with Duroc boars; however, <jats:styled-content style="fixed-case">PSE</jats:styled-content> (pale, soft and exudative) meat was frequently reported in the crossbred pigs, and the underlying reason remains unknown. We excluded the possibility of the well‐known causative mutations in <jats:italic><jats:styled-content style="fixed-case">RYR</jats:styled-content>1</jats:italic> and <jats:italic><jats:styled-content style="fixed-case">PRKAG</jats:styled-content>3</jats:italic> but identified the existence of an unfavorable allele of a splicing mutation (g.8283C>A) in <jats:italic><jats:styled-content style="fixed-case">PHKG</jats:styled-content>1</jats:italic> in two Duroc boars and three Duroc × Luchuan crossbred pigs with <jats:styled-content style="fixed-case">PSE</jats:styled-content> meat. An association analysis with 425 Duroc × Luchuan crossbred pigs revealed that the polymorphism of the splicing site of <jats:italic><jats:styled-content style="fixed-case">PHKG</jats:styled-content>1</jats:italic> has significant association with the ultimate meat <jats:styled-content style="fixed-case">pH</jats:styled-content> value (<jats:italic>P </jats:italic>=<jats:italic> </jats:italic>0.035) and color score (<jats:italic>P </jats:italic>=<jats:italic> </jats:italic>0.004). In addition, a strong cis‐<jats:styled-content style="fixed-case">eQTL</jats:styled-content> (expression <jats:styled-content style="fixed-case">QTL</jats:styled-content>) signal for the expression of <jats:italic><jats:styled-content style="fixed-case">PHKG</jats:styled-content>1</jats:italic> was identified in 189 Duroc × Luchuan crossbred pigs, and the splicing mutation was proven to be significantly associated with the expression of <jats:italic><jats:styled-content style="fixed-case">PHKG</jats:styled-content>1</jats:italic> (<jats:italic>P </jats:italic>=<jats:italic> </jats:italic>4.01e‐11). Furthermore, <jats:styled-content style="fixed-case">RNA</jats:styled-content>‐sequencing data analysis confirmed that 131 <jats:styled-content style="fixed-case">CC</jats:styled-content> homozygotes had only one transcript (T1), with <jats:styled-content style="fixed-case">FPKM</jats:styled-content> (fragments per kilobase of transcript per million) of 35.40 ± 7.28, and 58 <jats:styled-content style="fixed-case">CA</jats:styled-content> heterozygotes had two types of transcripts (T1 and T2), with <jats:styled-content style="fixed-case">FPKM</jats:styled-content> of 19.63 ± 5.11 and 9.20 ± 2.39 respectively. Based on the association and <jats:styled-content style="fixed-case">eQTL</jats:styled-content> analysis results, we concluded that <jats:styled-content style="fixed-case">PSE</jats:styled-content> meat in Duroc × Luchuan crossbred pigs is caused by the splicing mutation in <jats:italic><jats:styled-content style="fixed-case">PHKG</jats:styled-content>1</jats:italic>. Our findings further support the effect of the causative mutation in <jats:italic><jats:styled-content style="fixed-case">PHKG</jats:styled-content>1</jats:italic> on meat quality. The <jats:styled-content style="fixed-case">GEO</jats:styled-content> accession number for the data is <jats:styled-content style="fixed-case">GSE</jats:styled-content>124315.</jats:p> |
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Umfang: | 395-398 |
ISSN: |
0268-9146
1365-2052 |
DOI: | 10.1111/age.12807 |