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Cytokines, Osteoprotegerin, and RANKL In Vitro and Histomorphometric Indices of Bone Turnover in Patients With Different Bone Diseases
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| Zeitschriftentitel: | Journal of Bone and Mineral Research |
|---|---|
| Personen und Körperschaften: | , , , , , , , |
| In: | Journal of Bone and Mineral Research, 18, 2003, 3, S. 529-538 |
| Medientyp: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Oxford University Press (OUP)
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| Schlagwörter: |
| author_facet |
Siggelkow, Heide Eidner, Thorsten Lehmann, Gabriele Viereck, Volker Raddatz, Dirk Munzel, Ullrich Hein, Gert Hüfner, Michael Siggelkow, Heide Eidner, Thorsten Lehmann, Gabriele Viereck, Volker Raddatz, Dirk Munzel, Ullrich Hein, Gert Hüfner, Michael |
|---|---|
| author |
Siggelkow, Heide Eidner, Thorsten Lehmann, Gabriele Viereck, Volker Raddatz, Dirk Munzel, Ullrich Hein, Gert Hüfner, Michael |
| spellingShingle |
Siggelkow, Heide Eidner, Thorsten Lehmann, Gabriele Viereck, Volker Raddatz, Dirk Munzel, Ullrich Hein, Gert Hüfner, Michael Journal of Bone and Mineral Research Cytokines, Osteoprotegerin, and RANKL In Vitro and Histomorphometric Indices of Bone Turnover in Patients With Different Bone Diseases Orthopedics and Sports Medicine Endocrinology, Diabetes and Metabolism |
| author_sort |
siggelkow, heide |
| spelling |
Siggelkow, Heide Eidner, Thorsten Lehmann, Gabriele Viereck, Volker Raddatz, Dirk Munzel, Ullrich Hein, Gert Hüfner, Michael 0884-0431 1523-4681 Oxford University Press (OUP) Orthopedics and Sports Medicine Endocrinology, Diabetes and Metabolism http://dx.doi.org/10.1359/jbmr.2003.18.3.529 <jats:title>Abstract</jats:title> <jats:p>Cytokines are supposed to play an essential role in the regulation of the bone metabolic unit. However, information on cytokine production of primary human osteoblasts from patients with metabolic bone disease is scarce, and few attempts have been made to correlate such data to histomorphometric parameters of individual patients. We investigated 11 patients with metabolic bone disease referred to our outpatient department for bone biopsy and analyzed interleukin (IL)-1, IL-6, and TNF-α protein release and gene expression in primary osteoblast cultures. Compared with four controls, five patients showed normal cytokine protein release, whereas six patients showed much higher levels of interleukin-6 (26-fold) and TNF-α (84-fold). All three cytokines were strongly correlated concerning gene expression and/or protein levels (r = 0.72–0.96). Histomorphometric analysis of the bone samples showed that eroded surface (ES/BS) as a parameter of bone resorption was significantly associated with TNF-α. In addition, RANKL gene expression was positively associated with ES/BS and osteoclast surface (Oc.S/BS). Finally, the formation parameters osteoid volume and osteoid surface were negatively associated with TNF-α. In conclusion, in an in vitro-ex vivo model of bone cells obtained from a group of 11 patients with different forms of metabolic bone disease, cytokine release in conditioned medium was significantly associated with bone resorption and bone formation, as quantified by histomorphometry. TNF-α seemed to be the more important cytokine; its effect on bone resorption could be mediated by RANKL.</jats:p> Cytokines, Osteoprotegerin, and RANKL In Vitro and Histomorphometric Indices of Bone Turnover in Patients With Different Bone Diseases Journal of Bone and Mineral Research |
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| title |
Cytokines, Osteoprotegerin, and RANKL In Vitro and Histomorphometric Indices of Bone Turnover in Patients With Different Bone Diseases |
| title_unstemmed |
Cytokines, Osteoprotegerin, and RANKL In Vitro and Histomorphometric Indices of Bone Turnover in Patients With Different Bone Diseases |
| title_full |
Cytokines, Osteoprotegerin, and RANKL In Vitro and Histomorphometric Indices of Bone Turnover in Patients With Different Bone Diseases |
| title_fullStr |
Cytokines, Osteoprotegerin, and RANKL In Vitro and Histomorphometric Indices of Bone Turnover in Patients With Different Bone Diseases |
| title_full_unstemmed |
Cytokines, Osteoprotegerin, and RANKL In Vitro and Histomorphometric Indices of Bone Turnover in Patients With Different Bone Diseases |
| title_short |
Cytokines, Osteoprotegerin, and RANKL In Vitro and Histomorphometric Indices of Bone Turnover in Patients With Different Bone Diseases |
| title_sort |
cytokines, osteoprotegerin, and rankl in vitro and histomorphometric indices of bone turnover in patients with different bone diseases |
| topic |
Orthopedics and Sports Medicine Endocrinology, Diabetes and Metabolism |
| url |
http://dx.doi.org/10.1359/jbmr.2003.18.3.529 |
| publishDate |
2003 |
| physical |
529-538 |
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<jats:title>Abstract</jats:title>
<jats:p>Cytokines are supposed to play an essential role in the regulation of the bone metabolic unit. However, information on cytokine production of primary human osteoblasts from patients with metabolic bone disease is scarce, and few attempts have been made to correlate such data to histomorphometric parameters of individual patients. We investigated 11 patients with metabolic bone disease referred to our outpatient department for bone biopsy and analyzed interleukin (IL)-1, IL-6, and TNF-α protein release and gene expression in primary osteoblast cultures. Compared with four controls, five patients showed normal cytokine protein release, whereas six patients showed much higher levels of interleukin-6 (26-fold) and TNF-α (84-fold). All three cytokines were strongly correlated concerning gene expression and/or protein levels (r = 0.72–0.96). Histomorphometric analysis of the bone samples showed that eroded surface (ES/BS) as a parameter of bone resorption was significantly associated with TNF-α. In addition, RANKL gene expression was positively associated with ES/BS and osteoclast surface (Oc.S/BS). Finally, the formation parameters osteoid volume and osteoid surface were negatively associated with TNF-α. In conclusion, in an in vitro-ex vivo model of bone cells obtained from a group of 11 patients with different forms of metabolic bone disease, cytokine release in conditioned medium was significantly associated with bone resorption and bone formation, as quantified by histomorphometry. TNF-α seemed to be the more important cytokine; its effect on bone resorption could be mediated by RANKL.</jats:p> |
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| author | Siggelkow, Heide, Eidner, Thorsten, Lehmann, Gabriele, Viereck, Volker, Raddatz, Dirk, Munzel, Ullrich, Hein, Gert, Hüfner, Michael |
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| description | <jats:title>Abstract</jats:title> <jats:p>Cytokines are supposed to play an essential role in the regulation of the bone metabolic unit. However, information on cytokine production of primary human osteoblasts from patients with metabolic bone disease is scarce, and few attempts have been made to correlate such data to histomorphometric parameters of individual patients. We investigated 11 patients with metabolic bone disease referred to our outpatient department for bone biopsy and analyzed interleukin (IL)-1, IL-6, and TNF-α protein release and gene expression in primary osteoblast cultures. Compared with four controls, five patients showed normal cytokine protein release, whereas six patients showed much higher levels of interleukin-6 (26-fold) and TNF-α (84-fold). All three cytokines were strongly correlated concerning gene expression and/or protein levels (r = 0.72–0.96). Histomorphometric analysis of the bone samples showed that eroded surface (ES/BS) as a parameter of bone resorption was significantly associated with TNF-α. In addition, RANKL gene expression was positively associated with ES/BS and osteoclast surface (Oc.S/BS). Finally, the formation parameters osteoid volume and osteoid surface were negatively associated with TNF-α. In conclusion, in an in vitro-ex vivo model of bone cells obtained from a group of 11 patients with different forms of metabolic bone disease, cytokine release in conditioned medium was significantly associated with bone resorption and bone formation, as quantified by histomorphometry. TNF-α seemed to be the more important cytokine; its effect on bone resorption could be mediated by RANKL.</jats:p> |
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| institution | DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1 |
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| spelling | Siggelkow, Heide Eidner, Thorsten Lehmann, Gabriele Viereck, Volker Raddatz, Dirk Munzel, Ullrich Hein, Gert Hüfner, Michael 0884-0431 1523-4681 Oxford University Press (OUP) Orthopedics and Sports Medicine Endocrinology, Diabetes and Metabolism http://dx.doi.org/10.1359/jbmr.2003.18.3.529 <jats:title>Abstract</jats:title> <jats:p>Cytokines are supposed to play an essential role in the regulation of the bone metabolic unit. However, information on cytokine production of primary human osteoblasts from patients with metabolic bone disease is scarce, and few attempts have been made to correlate such data to histomorphometric parameters of individual patients. We investigated 11 patients with metabolic bone disease referred to our outpatient department for bone biopsy and analyzed interleukin (IL)-1, IL-6, and TNF-α protein release and gene expression in primary osteoblast cultures. Compared with four controls, five patients showed normal cytokine protein release, whereas six patients showed much higher levels of interleukin-6 (26-fold) and TNF-α (84-fold). All three cytokines were strongly correlated concerning gene expression and/or protein levels (r = 0.72–0.96). Histomorphometric analysis of the bone samples showed that eroded surface (ES/BS) as a parameter of bone resorption was significantly associated with TNF-α. In addition, RANKL gene expression was positively associated with ES/BS and osteoclast surface (Oc.S/BS). Finally, the formation parameters osteoid volume and osteoid surface were negatively associated with TNF-α. In conclusion, in an in vitro-ex vivo model of bone cells obtained from a group of 11 patients with different forms of metabolic bone disease, cytokine release in conditioned medium was significantly associated with bone resorption and bone formation, as quantified by histomorphometry. TNF-α seemed to be the more important cytokine; its effect on bone resorption could be mediated by RANKL.</jats:p> Cytokines, Osteoprotegerin, and RANKL In Vitro and Histomorphometric Indices of Bone Turnover in Patients With Different Bone Diseases Journal of Bone and Mineral Research |
| spellingShingle | Siggelkow, Heide, Eidner, Thorsten, Lehmann, Gabriele, Viereck, Volker, Raddatz, Dirk, Munzel, Ullrich, Hein, Gert, Hüfner, Michael, Journal of Bone and Mineral Research, Cytokines, Osteoprotegerin, and RANKL In Vitro and Histomorphometric Indices of Bone Turnover in Patients With Different Bone Diseases, Orthopedics and Sports Medicine, Endocrinology, Diabetes and Metabolism |
| title | Cytokines, Osteoprotegerin, and RANKL In Vitro and Histomorphometric Indices of Bone Turnover in Patients With Different Bone Diseases |
| title_full | Cytokines, Osteoprotegerin, and RANKL In Vitro and Histomorphometric Indices of Bone Turnover in Patients With Different Bone Diseases |
| title_fullStr | Cytokines, Osteoprotegerin, and RANKL In Vitro and Histomorphometric Indices of Bone Turnover in Patients With Different Bone Diseases |
| title_full_unstemmed | Cytokines, Osteoprotegerin, and RANKL In Vitro and Histomorphometric Indices of Bone Turnover in Patients With Different Bone Diseases |
| title_short | Cytokines, Osteoprotegerin, and RANKL In Vitro and Histomorphometric Indices of Bone Turnover in Patients With Different Bone Diseases |
| title_sort | cytokines, osteoprotegerin, and rankl in vitro and histomorphometric indices of bone turnover in patients with different bone diseases |
| title_unstemmed | Cytokines, Osteoprotegerin, and RANKL In Vitro and Histomorphometric Indices of Bone Turnover in Patients With Different Bone Diseases |
| topic | Orthopedics and Sports Medicine, Endocrinology, Diabetes and Metabolism |
| url | http://dx.doi.org/10.1359/jbmr.2003.18.3.529 |