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Bacilli glutamate dehydrogenases diverged via coevolution of transcription and enzyme regulation
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Zeitschriftentitel: | EMBO reports |
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Personen und Körperschaften: | , , , , |
In: | EMBO reports, 18, 2017, 7, S. 1139-1149 |
Medientyp: | E-Article |
Sprache: | Englisch |
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Springer Science and Business Media LLC
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author_facet |
Noda‐Garcia, Lianet Romero Romero, Maria Luisa Longo, Liam M Kolodkin‐Gal, Ilana Tawfik, Dan S Noda‐Garcia, Lianet Romero Romero, Maria Luisa Longo, Liam M Kolodkin‐Gal, Ilana Tawfik, Dan S |
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author |
Noda‐Garcia, Lianet Romero Romero, Maria Luisa Longo, Liam M Kolodkin‐Gal, Ilana Tawfik, Dan S |
spellingShingle |
Noda‐Garcia, Lianet Romero Romero, Maria Luisa Longo, Liam M Kolodkin‐Gal, Ilana Tawfik, Dan S EMBO reports Bacilli glutamate dehydrogenases diverged via coevolution of transcription and enzyme regulation Genetics Molecular Biology Biochemistry |
author_sort |
noda‐garcia, lianet |
spelling |
Noda‐Garcia, Lianet Romero Romero, Maria Luisa Longo, Liam M Kolodkin‐Gal, Ilana Tawfik, Dan S 1469-221X 1469-3178 Springer Science and Business Media LLC Genetics Molecular Biology Biochemistry http://dx.doi.org/10.15252/embr.201743990 <jats:title>Abstract</jats:title><jats:p>The linkage between regulatory elements of transcription, such as promoters, and their protein products is central to gene function. Promoter–protein coevolution is therefore expected, but rarely observed, and the manner by which these two regulatory levels are linked remains largely unknown. We study glutamate dehydrogenase—a hub of carbon and nitrogen metabolism. In <jats:italic>Bacillus subtilis</jats:italic>, two paralogues exist: GudB is constitutively transcribed whereas RocG is tightly regulated. In their active, oligomeric states, both enzymes show similar enzymatic rates. However, swaps of enzymes and promoters cause severe fitness losses, thus indicating promoter–enzyme coevolution. Characterization of the proteins shows that, compared to RocG, GudB's enzymatic activity is highly dependent on glutamate and <jats:styled-content style="fixed-case">pH</jats:styled-content>. Promoter–enzyme swaps therefore result in excessive glutamate degradation when expressing a constitutive enzyme under a constitutive promoter, or insufficient activity when both the enzyme and its promoter are tightly regulated. Coevolution of transcriptional and enzymatic regulation therefore underlies paralogue‐specific spatio‐temporal control, especially under diverse growth conditions.</jats:p> <i>Bacilli</i> glutamate dehydrogenases diverged via coevolution of transcription and enzyme regulation EMBO reports |
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Springer Science and Business Media LLC |
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title |
Bacilli glutamate dehydrogenases diverged via coevolution of transcription and enzyme regulation |
title_unstemmed |
Bacilli glutamate dehydrogenases diverged via coevolution of transcription and enzyme regulation |
title_full |
Bacilli glutamate dehydrogenases diverged via coevolution of transcription and enzyme regulation |
title_fullStr |
Bacilli glutamate dehydrogenases diverged via coevolution of transcription and enzyme regulation |
title_full_unstemmed |
Bacilli glutamate dehydrogenases diverged via coevolution of transcription and enzyme regulation |
title_short |
Bacilli glutamate dehydrogenases diverged via coevolution of transcription and enzyme regulation |
title_sort |
<i>bacilli</i> glutamate dehydrogenases diverged via coevolution of transcription and enzyme regulation |
topic |
Genetics Molecular Biology Biochemistry |
url |
http://dx.doi.org/10.15252/embr.201743990 |
publishDate |
2017 |
physical |
1139-1149 |
description |
<jats:title>Abstract</jats:title><jats:p>The linkage between regulatory elements of transcription, such as promoters, and their protein products is central to gene function. Promoter–protein coevolution is therefore expected, but rarely observed, and the manner by which these two regulatory levels are linked remains largely unknown. We study glutamate dehydrogenase—a hub of carbon and nitrogen metabolism. In <jats:italic>Bacillus subtilis</jats:italic>, two paralogues exist: GudB is constitutively transcribed whereas RocG is tightly regulated. In their active, oligomeric states, both enzymes show similar enzymatic rates. However, swaps of enzymes and promoters cause severe fitness losses, thus indicating promoter–enzyme coevolution. Characterization of the proteins shows that, compared to RocG, GudB's enzymatic activity is highly dependent on glutamate and <jats:styled-content style="fixed-case">pH</jats:styled-content>. Promoter–enzyme swaps therefore result in excessive glutamate degradation when expressing a constitutive enzyme under a constitutive promoter, or insufficient activity when both the enzyme and its promoter are tightly regulated. Coevolution of transcriptional and enzymatic regulation therefore underlies paralogue‐specific spatio‐temporal control, especially under diverse growth conditions.</jats:p> |
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author | Noda‐Garcia, Lianet, Romero Romero, Maria Luisa, Longo, Liam M, Kolodkin‐Gal, Ilana, Tawfik, Dan S |
author_facet | Noda‐Garcia, Lianet, Romero Romero, Maria Luisa, Longo, Liam M, Kolodkin‐Gal, Ilana, Tawfik, Dan S, Noda‐Garcia, Lianet, Romero Romero, Maria Luisa, Longo, Liam M, Kolodkin‐Gal, Ilana, Tawfik, Dan S |
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description | <jats:title>Abstract</jats:title><jats:p>The linkage between regulatory elements of transcription, such as promoters, and their protein products is central to gene function. Promoter–protein coevolution is therefore expected, but rarely observed, and the manner by which these two regulatory levels are linked remains largely unknown. We study glutamate dehydrogenase—a hub of carbon and nitrogen metabolism. In <jats:italic>Bacillus subtilis</jats:italic>, two paralogues exist: GudB is constitutively transcribed whereas RocG is tightly regulated. In their active, oligomeric states, both enzymes show similar enzymatic rates. However, swaps of enzymes and promoters cause severe fitness losses, thus indicating promoter–enzyme coevolution. Characterization of the proteins shows that, compared to RocG, GudB's enzymatic activity is highly dependent on glutamate and <jats:styled-content style="fixed-case">pH</jats:styled-content>. Promoter–enzyme swaps therefore result in excessive glutamate degradation when expressing a constitutive enzyme under a constitutive promoter, or insufficient activity when both the enzyme and its promoter are tightly regulated. Coevolution of transcriptional and enzymatic regulation therefore underlies paralogue‐specific spatio‐temporal control, especially under diverse growth conditions.</jats:p> |
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spelling | Noda‐Garcia, Lianet Romero Romero, Maria Luisa Longo, Liam M Kolodkin‐Gal, Ilana Tawfik, Dan S 1469-221X 1469-3178 Springer Science and Business Media LLC Genetics Molecular Biology Biochemistry http://dx.doi.org/10.15252/embr.201743990 <jats:title>Abstract</jats:title><jats:p>The linkage between regulatory elements of transcription, such as promoters, and their protein products is central to gene function. Promoter–protein coevolution is therefore expected, but rarely observed, and the manner by which these two regulatory levels are linked remains largely unknown. We study glutamate dehydrogenase—a hub of carbon and nitrogen metabolism. In <jats:italic>Bacillus subtilis</jats:italic>, two paralogues exist: GudB is constitutively transcribed whereas RocG is tightly regulated. In their active, oligomeric states, both enzymes show similar enzymatic rates. However, swaps of enzymes and promoters cause severe fitness losses, thus indicating promoter–enzyme coevolution. Characterization of the proteins shows that, compared to RocG, GudB's enzymatic activity is highly dependent on glutamate and <jats:styled-content style="fixed-case">pH</jats:styled-content>. Promoter–enzyme swaps therefore result in excessive glutamate degradation when expressing a constitutive enzyme under a constitutive promoter, or insufficient activity when both the enzyme and its promoter are tightly regulated. Coevolution of transcriptional and enzymatic regulation therefore underlies paralogue‐specific spatio‐temporal control, especially under diverse growth conditions.</jats:p> <i>Bacilli</i> glutamate dehydrogenases diverged via coevolution of transcription and enzyme regulation EMBO reports |
spellingShingle | Noda‐Garcia, Lianet, Romero Romero, Maria Luisa, Longo, Liam M, Kolodkin‐Gal, Ilana, Tawfik, Dan S, EMBO reports, Bacilli glutamate dehydrogenases diverged via coevolution of transcription and enzyme regulation, Genetics, Molecular Biology, Biochemistry |
title | Bacilli glutamate dehydrogenases diverged via coevolution of transcription and enzyme regulation |
title_full | Bacilli glutamate dehydrogenases diverged via coevolution of transcription and enzyme regulation |
title_fullStr | Bacilli glutamate dehydrogenases diverged via coevolution of transcription and enzyme regulation |
title_full_unstemmed | Bacilli glutamate dehydrogenases diverged via coevolution of transcription and enzyme regulation |
title_short | Bacilli glutamate dehydrogenases diverged via coevolution of transcription and enzyme regulation |
title_sort | <i>bacilli</i> glutamate dehydrogenases diverged via coevolution of transcription and enzyme regulation |
title_unstemmed | Bacilli glutamate dehydrogenases diverged via coevolution of transcription and enzyme regulation |
topic | Genetics, Molecular Biology, Biochemistry |
url | http://dx.doi.org/10.15252/embr.201743990 |