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Bacilli glutamate dehydrogenases diverged via coevolution of transcription and enzyme regulation

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Zeitschriftentitel: EMBO reports
Personen und Körperschaften: Noda‐Garcia, Lianet, Romero Romero, Maria Luisa, Longo, Liam M, Kolodkin‐Gal, Ilana, Tawfik, Dan S
In: EMBO reports, 18, 2017, 7, S. 1139-1149
Medientyp: E-Article
Sprache: Englisch
veröffentlicht:
Springer Science and Business Media LLC
Schlagwörter:
author_facet Noda‐Garcia, Lianet
Romero Romero, Maria Luisa
Longo, Liam M
Kolodkin‐Gal, Ilana
Tawfik, Dan S
Noda‐Garcia, Lianet
Romero Romero, Maria Luisa
Longo, Liam M
Kolodkin‐Gal, Ilana
Tawfik, Dan S
author Noda‐Garcia, Lianet
Romero Romero, Maria Luisa
Longo, Liam M
Kolodkin‐Gal, Ilana
Tawfik, Dan S
spellingShingle Noda‐Garcia, Lianet
Romero Romero, Maria Luisa
Longo, Liam M
Kolodkin‐Gal, Ilana
Tawfik, Dan S
EMBO reports
Bacilli glutamate dehydrogenases diverged via coevolution of transcription and enzyme regulation
Genetics
Molecular Biology
Biochemistry
author_sort noda‐garcia, lianet
spelling Noda‐Garcia, Lianet Romero Romero, Maria Luisa Longo, Liam M Kolodkin‐Gal, Ilana Tawfik, Dan S 1469-221X 1469-3178 Springer Science and Business Media LLC Genetics Molecular Biology Biochemistry http://dx.doi.org/10.15252/embr.201743990 <jats:title>Abstract</jats:title><jats:p>The linkage between regulatory elements of transcription, such as promoters, and their protein products is central to gene function. Promoter–protein coevolution is therefore expected, but rarely observed, and the manner by which these two regulatory levels are linked remains largely unknown. We study glutamate dehydrogenase—a hub of carbon and nitrogen metabolism. In <jats:italic>Bacillus subtilis</jats:italic>, two paralogues exist: GudB is constitutively transcribed whereas RocG is tightly regulated. In their active, oligomeric states, both enzymes show similar enzymatic rates. However, swaps of enzymes and promoters cause severe fitness losses, thus indicating promoter–enzyme coevolution. Characterization of the proteins shows that, compared to RocG, GudB's enzymatic activity is highly dependent on glutamate and <jats:styled-content style="fixed-case">pH</jats:styled-content>. Promoter–enzyme swaps therefore result in excessive glutamate degradation when expressing a constitutive enzyme under a constitutive promoter, or insufficient activity when both the enzyme and its promoter are tightly regulated. Coevolution of transcriptional and enzymatic regulation therefore underlies paralogue‐specific spatio‐temporal control, especially under diverse growth conditions.</jats:p> <i>Bacilli</i> glutamate dehydrogenases diverged via coevolution of transcription and enzyme regulation EMBO reports
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title Bacilli glutamate dehydrogenases diverged via coevolution of transcription and enzyme regulation
title_unstemmed Bacilli glutamate dehydrogenases diverged via coevolution of transcription and enzyme regulation
title_full Bacilli glutamate dehydrogenases diverged via coevolution of transcription and enzyme regulation
title_fullStr Bacilli glutamate dehydrogenases diverged via coevolution of transcription and enzyme regulation
title_full_unstemmed Bacilli glutamate dehydrogenases diverged via coevolution of transcription and enzyme regulation
title_short Bacilli glutamate dehydrogenases diverged via coevolution of transcription and enzyme regulation
title_sort <i>bacilli</i> glutamate dehydrogenases diverged via coevolution of transcription and enzyme regulation
topic Genetics
Molecular Biology
Biochemistry
url http://dx.doi.org/10.15252/embr.201743990
publishDate 2017
physical 1139-1149
description <jats:title>Abstract</jats:title><jats:p>The linkage between regulatory elements of transcription, such as promoters, and their protein products is central to gene function. Promoter–protein coevolution is therefore expected, but rarely observed, and the manner by which these two regulatory levels are linked remains largely unknown. We study glutamate dehydrogenase—a hub of carbon and nitrogen metabolism. In <jats:italic>Bacillus subtilis</jats:italic>, two paralogues exist: GudB is constitutively transcribed whereas RocG is tightly regulated. In their active, oligomeric states, both enzymes show similar enzymatic rates. However, swaps of enzymes and promoters cause severe fitness losses, thus indicating promoter–enzyme coevolution. Characterization of the proteins shows that, compared to RocG, GudB's enzymatic activity is highly dependent on glutamate and <jats:styled-content style="fixed-case">pH</jats:styled-content>. Promoter–enzyme swaps therefore result in excessive glutamate degradation when expressing a constitutive enzyme under a constitutive promoter, or insufficient activity when both the enzyme and its promoter are tightly regulated. Coevolution of transcriptional and enzymatic regulation therefore underlies paralogue‐specific spatio‐temporal control, especially under diverse growth conditions.</jats:p>
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author Noda‐Garcia, Lianet, Romero Romero, Maria Luisa, Longo, Liam M, Kolodkin‐Gal, Ilana, Tawfik, Dan S
author_facet Noda‐Garcia, Lianet, Romero Romero, Maria Luisa, Longo, Liam M, Kolodkin‐Gal, Ilana, Tawfik, Dan S, Noda‐Garcia, Lianet, Romero Romero, Maria Luisa, Longo, Liam M, Kolodkin‐Gal, Ilana, Tawfik, Dan S
author_sort noda‐garcia, lianet
container_issue 7
container_start_page 1139
container_title EMBO reports
container_volume 18
description <jats:title>Abstract</jats:title><jats:p>The linkage between regulatory elements of transcription, such as promoters, and their protein products is central to gene function. Promoter–protein coevolution is therefore expected, but rarely observed, and the manner by which these two regulatory levels are linked remains largely unknown. We study glutamate dehydrogenase—a hub of carbon and nitrogen metabolism. In <jats:italic>Bacillus subtilis</jats:italic>, two paralogues exist: GudB is constitutively transcribed whereas RocG is tightly regulated. In their active, oligomeric states, both enzymes show similar enzymatic rates. However, swaps of enzymes and promoters cause severe fitness losses, thus indicating promoter–enzyme coevolution. Characterization of the proteins shows that, compared to RocG, GudB's enzymatic activity is highly dependent on glutamate and <jats:styled-content style="fixed-case">pH</jats:styled-content>. Promoter–enzyme swaps therefore result in excessive glutamate degradation when expressing a constitutive enzyme under a constitutive promoter, or insufficient activity when both the enzyme and its promoter are tightly regulated. Coevolution of transcriptional and enzymatic regulation therefore underlies paralogue‐specific spatio‐temporal control, especially under diverse growth conditions.</jats:p>
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institution DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161
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spelling Noda‐Garcia, Lianet Romero Romero, Maria Luisa Longo, Liam M Kolodkin‐Gal, Ilana Tawfik, Dan S 1469-221X 1469-3178 Springer Science and Business Media LLC Genetics Molecular Biology Biochemistry http://dx.doi.org/10.15252/embr.201743990 <jats:title>Abstract</jats:title><jats:p>The linkage between regulatory elements of transcription, such as promoters, and their protein products is central to gene function. Promoter–protein coevolution is therefore expected, but rarely observed, and the manner by which these two regulatory levels are linked remains largely unknown. We study glutamate dehydrogenase—a hub of carbon and nitrogen metabolism. In <jats:italic>Bacillus subtilis</jats:italic>, two paralogues exist: GudB is constitutively transcribed whereas RocG is tightly regulated. In their active, oligomeric states, both enzymes show similar enzymatic rates. However, swaps of enzymes and promoters cause severe fitness losses, thus indicating promoter–enzyme coevolution. Characterization of the proteins shows that, compared to RocG, GudB's enzymatic activity is highly dependent on glutamate and <jats:styled-content style="fixed-case">pH</jats:styled-content>. Promoter–enzyme swaps therefore result in excessive glutamate degradation when expressing a constitutive enzyme under a constitutive promoter, or insufficient activity when both the enzyme and its promoter are tightly regulated. Coevolution of transcriptional and enzymatic regulation therefore underlies paralogue‐specific spatio‐temporal control, especially under diverse growth conditions.</jats:p> <i>Bacilli</i> glutamate dehydrogenases diverged via coevolution of transcription and enzyme regulation EMBO reports
spellingShingle Noda‐Garcia, Lianet, Romero Romero, Maria Luisa, Longo, Liam M, Kolodkin‐Gal, Ilana, Tawfik, Dan S, EMBO reports, Bacilli glutamate dehydrogenases diverged via coevolution of transcription and enzyme regulation, Genetics, Molecular Biology, Biochemistry
title Bacilli glutamate dehydrogenases diverged via coevolution of transcription and enzyme regulation
title_full Bacilli glutamate dehydrogenases diverged via coevolution of transcription and enzyme regulation
title_fullStr Bacilli glutamate dehydrogenases diverged via coevolution of transcription and enzyme regulation
title_full_unstemmed Bacilli glutamate dehydrogenases diverged via coevolution of transcription and enzyme regulation
title_short Bacilli glutamate dehydrogenases diverged via coevolution of transcription and enzyme regulation
title_sort <i>bacilli</i> glutamate dehydrogenases diverged via coevolution of transcription and enzyme regulation
title_unstemmed Bacilli glutamate dehydrogenases diverged via coevolution of transcription and enzyme regulation
topic Genetics, Molecular Biology, Biochemistry
url http://dx.doi.org/10.15252/embr.201743990