Eintrag weiter verarbeiten
Buchumschlag von IMAGING OF FLUOROPHORES IN CHROMATOGRAPHIC BEADS, RECONSTRUCTION OF RADIAL DENSITY DISTRIBUTIONS AND CHARACTERISATION OF PROTEIN UPTAKING PROCESSES
Verfügbar über Open Access

IMAGING OF FLUOROPHORES IN CHROMATOGRAPHIC BEADS, RECONSTRUCTION OF RADIAL DENSITY DISTRIBUTIONS AND CHARACTERISATION OF PROTEIN UPTAKING PROCESSES

Gespeichert in:

Bibliographische Detailangaben
Zeitschriftentitel: Image Analysis & Stereology
Personen und Körperschaften: Stanislawski, Bernd, Schmit, Elodie, Ohser, Joachim
In: Image Analysis & Stereology, 29, 2010, 3, S. 181
Medientyp: E-Article
Sprache: Unbestimmt
veröffentlicht:
Slovenian Society for Stereology and Quantitative Image Analysis
Schlagwörter:
author_facet Stanislawski, Bernd
Schmit, Elodie
Ohser, Joachim
Stanislawski, Bernd
Schmit, Elodie
Ohser, Joachim
author Stanislawski, Bernd
Schmit, Elodie
Ohser, Joachim
spellingShingle Stanislawski, Bernd
Schmit, Elodie
Ohser, Joachim
Image Analysis & Stereology
IMAGING OF FLUOROPHORES IN CHROMATOGRAPHIC BEADS, RECONSTRUCTION OF RADIAL DENSITY DISTRIBUTIONS AND CHARACTERISATION OF PROTEIN UPTAKING PROCESSES
Computer Vision and Pattern Recognition
Acoustics and Ultrasonics
Radiology, Nuclear Medicine and imaging
Instrumentation
Materials Science (miscellaneous)
General Mathematics
Signal Processing
Biotechnology
author_sort stanislawski, bernd
spelling Stanislawski, Bernd Schmit, Elodie Ohser, Joachim 1854-5165 1580-3139 Slovenian Society for Stereology and Quantitative Image Analysis Computer Vision and Pattern Recognition Acoustics and Ultrasonics Radiology, Nuclear Medicine and imaging Instrumentation Materials Science (miscellaneous) General Mathematics Signal Processing Biotechnology http://dx.doi.org/10.5566/ias.v29.p181-189 <jats:p>A new adjustment calculus is presented to determine the true intraparticle distribution of bound protein within chromatographic beads from confocal fluorescence slice series. The calculus does not require knowledge about optical properties of different chromatographic materials like refractive index and turbidity, but it depends on a parameter which can be adjusted interactively. The algorithm is of complexity O(n) where n is the pixel number. From the reconstructed data we compute the parameters of the protein uptaking process using a model-based approach. It is demonstrated that the protein uptaking rates of the beads strongly dependent on the conditions of the fluid phase influencing the strength of protein surface interaction.</jats:p> IMAGING OF FLUOROPHORES IN CHROMATOGRAPHIC BEADS, RECONSTRUCTION OF RADIAL DENSITY DISTRIBUTIONS AND CHARACTERISATION OF PROTEIN UPTAKING PROCESSES Image Analysis & Stereology
doi_str_mv 10.5566/ias.v29.p181-189
facet_avail Online
Free
finc_class_facet Informatik
Physik
Allgemeines
Technik
Biologie
format ElectronicArticle
fullrecord blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuNTU2Ni9pYXMudjI5LnAxODEtMTg5
id ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuNTU2Ni9pYXMudjI5LnAxODEtMTg5
institution DE-D275
DE-Bn3
DE-Brt1
DE-Zwi2
DE-D161
DE-Gla1
DE-Zi4
DE-15
DE-Pl11
DE-Rs1
DE-105
DE-14
DE-Ch1
DE-L229
imprint Slovenian Society for Stereology and Quantitative Image Analysis, 2010
imprint_str_mv Slovenian Society for Stereology and Quantitative Image Analysis, 2010
issn 1854-5165
1580-3139
issn_str_mv 1854-5165
1580-3139
language Undetermined
mega_collection Slovenian Society for Stereology and Quantitative Image Analysis (CrossRef)
match_str stanislawski2010imagingoffluorophoresinchromatographicbeadsreconstructionofradialdensitydistributionsandcharacterisationofproteinuptakingprocesses
publishDateSort 2010
publisher Slovenian Society for Stereology and Quantitative Image Analysis
recordtype ai
record_format ai
series Image Analysis & Stereology
source_id 49
title IMAGING OF FLUOROPHORES IN CHROMATOGRAPHIC BEADS, RECONSTRUCTION OF RADIAL DENSITY DISTRIBUTIONS AND CHARACTERISATION OF PROTEIN UPTAKING PROCESSES
title_unstemmed IMAGING OF FLUOROPHORES IN CHROMATOGRAPHIC BEADS, RECONSTRUCTION OF RADIAL DENSITY DISTRIBUTIONS AND CHARACTERISATION OF PROTEIN UPTAKING PROCESSES
title_full IMAGING OF FLUOROPHORES IN CHROMATOGRAPHIC BEADS, RECONSTRUCTION OF RADIAL DENSITY DISTRIBUTIONS AND CHARACTERISATION OF PROTEIN UPTAKING PROCESSES
title_fullStr IMAGING OF FLUOROPHORES IN CHROMATOGRAPHIC BEADS, RECONSTRUCTION OF RADIAL DENSITY DISTRIBUTIONS AND CHARACTERISATION OF PROTEIN UPTAKING PROCESSES
title_full_unstemmed IMAGING OF FLUOROPHORES IN CHROMATOGRAPHIC BEADS, RECONSTRUCTION OF RADIAL DENSITY DISTRIBUTIONS AND CHARACTERISATION OF PROTEIN UPTAKING PROCESSES
title_short IMAGING OF FLUOROPHORES IN CHROMATOGRAPHIC BEADS, RECONSTRUCTION OF RADIAL DENSITY DISTRIBUTIONS AND CHARACTERISATION OF PROTEIN UPTAKING PROCESSES
title_sort imaging of fluorophores in chromatographic beads, reconstruction of radial density distributions and characterisation of protein uptaking processes
topic Computer Vision and Pattern Recognition
Acoustics and Ultrasonics
Radiology, Nuclear Medicine and imaging
Instrumentation
Materials Science (miscellaneous)
General Mathematics
Signal Processing
Biotechnology
url http://dx.doi.org/10.5566/ias.v29.p181-189
publishDate 2010
physical 181
description <jats:p>A new adjustment calculus is presented to determine the true intraparticle distribution of bound protein within chromatographic beads from confocal fluorescence slice series. The calculus does not require knowledge about optical properties of different chromatographic materials like refractive index and turbidity, but it depends on a parameter which can be adjusted interactively. The algorithm is of complexity O(n) where n is the pixel number. From the reconstructed data we compute the parameters of the protein uptaking process using a model-based approach. It is demonstrated that the protein uptaking rates of the beads strongly dependent on the conditions of the fluid phase influencing the strength of protein surface interaction.</jats:p>
container_issue 3
container_start_page 0
container_title Image Analysis & Stereology
container_volume 29
format_de105 Article, E-Article
format_de14 Article, E-Article
format_de15 Article, E-Article
format_de520 Article, E-Article
format_de540 Article, E-Article
format_dech1 Article, E-Article
format_ded117 Article, E-Article
format_degla1 E-Article
format_del152 Buch
format_del189 Article, E-Article
format_dezi4 Article
format_dezwi2 Article, E-Article
format_finc Article, E-Article
format_nrw Article, E-Article
_version_ 1792333350270140419
geogr_code not assigned
last_indexed 2024-03-01T14:11:22.359Z
geogr_code_person not assigned
openURL url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=IMAGING+OF+FLUOROPHORES+IN+CHROMATOGRAPHIC+BEADS%2C+RECONSTRUCTION+OF+RADIAL+DENSITY+DISTRIBUTIONS+AND+CHARACTERISATION+OF+PROTEIN+UPTAKING+PROCESSES&rft.date=2010-11-01&genre=article&issn=1580-3139&volume=29&issue=3&pages=181&jtitle=Image+Analysis+%26+Stereology&atitle=IMAGING+OF+FLUOROPHORES+IN+CHROMATOGRAPHIC+BEADS%2C+RECONSTRUCTION+OF+RADIAL+DENSITY+DISTRIBUTIONS+AND+CHARACTERISATION+OF+PROTEIN+UPTAKING+PROCESSES&aulast=Ohser&aufirst=Joachim&rft_id=info%3Adoi%2F10.5566%2Fias.v29.p181-189&rft.language%5B0%5D=und
SOLR
_version_ 1792333350270140419
author Stanislawski, Bernd, Schmit, Elodie, Ohser, Joachim
author_facet Stanislawski, Bernd, Schmit, Elodie, Ohser, Joachim, Stanislawski, Bernd, Schmit, Elodie, Ohser, Joachim
author_sort stanislawski, bernd
container_issue 3
container_start_page 0
container_title Image Analysis & Stereology
container_volume 29
description <jats:p>A new adjustment calculus is presented to determine the true intraparticle distribution of bound protein within chromatographic beads from confocal fluorescence slice series. The calculus does not require knowledge about optical properties of different chromatographic materials like refractive index and turbidity, but it depends on a parameter which can be adjusted interactively. The algorithm is of complexity O(n) where n is the pixel number. From the reconstructed data we compute the parameters of the protein uptaking process using a model-based approach. It is demonstrated that the protein uptaking rates of the beads strongly dependent on the conditions of the fluid phase influencing the strength of protein surface interaction.</jats:p>
doi_str_mv 10.5566/ias.v29.p181-189
facet_avail Online, Free
finc_class_facet Informatik, Physik, Allgemeines, Technik, Biologie
format ElectronicArticle
format_de105 Article, E-Article
format_de14 Article, E-Article
format_de15 Article, E-Article
format_de520 Article, E-Article
format_de540 Article, E-Article
format_dech1 Article, E-Article
format_ded117 Article, E-Article
format_degla1 E-Article
format_del152 Buch
format_del189 Article, E-Article
format_dezi4 Article
format_dezwi2 Article, E-Article
format_finc Article, E-Article
format_nrw Article, E-Article
geogr_code not assigned
geogr_code_person not assigned
id ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuNTU2Ni9pYXMudjI5LnAxODEtMTg5
imprint Slovenian Society for Stereology and Quantitative Image Analysis, 2010
imprint_str_mv Slovenian Society for Stereology and Quantitative Image Analysis, 2010
institution DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229
issn 1854-5165, 1580-3139
issn_str_mv 1854-5165, 1580-3139
language Undetermined
last_indexed 2024-03-01T14:11:22.359Z
match_str stanislawski2010imagingoffluorophoresinchromatographicbeadsreconstructionofradialdensitydistributionsandcharacterisationofproteinuptakingprocesses
mega_collection Slovenian Society for Stereology and Quantitative Image Analysis (CrossRef)
physical 181
publishDate 2010
publishDateSort 2010
publisher Slovenian Society for Stereology and Quantitative Image Analysis
record_format ai
recordtype ai
series Image Analysis & Stereology
source_id 49
spelling Stanislawski, Bernd Schmit, Elodie Ohser, Joachim 1854-5165 1580-3139 Slovenian Society for Stereology and Quantitative Image Analysis Computer Vision and Pattern Recognition Acoustics and Ultrasonics Radiology, Nuclear Medicine and imaging Instrumentation Materials Science (miscellaneous) General Mathematics Signal Processing Biotechnology http://dx.doi.org/10.5566/ias.v29.p181-189 <jats:p>A new adjustment calculus is presented to determine the true intraparticle distribution of bound protein within chromatographic beads from confocal fluorescence slice series. The calculus does not require knowledge about optical properties of different chromatographic materials like refractive index and turbidity, but it depends on a parameter which can be adjusted interactively. The algorithm is of complexity O(n) where n is the pixel number. From the reconstructed data we compute the parameters of the protein uptaking process using a model-based approach. It is demonstrated that the protein uptaking rates of the beads strongly dependent on the conditions of the fluid phase influencing the strength of protein surface interaction.</jats:p> IMAGING OF FLUOROPHORES IN CHROMATOGRAPHIC BEADS, RECONSTRUCTION OF RADIAL DENSITY DISTRIBUTIONS AND CHARACTERISATION OF PROTEIN UPTAKING PROCESSES Image Analysis & Stereology
spellingShingle Stanislawski, Bernd, Schmit, Elodie, Ohser, Joachim, Image Analysis & Stereology, IMAGING OF FLUOROPHORES IN CHROMATOGRAPHIC BEADS, RECONSTRUCTION OF RADIAL DENSITY DISTRIBUTIONS AND CHARACTERISATION OF PROTEIN UPTAKING PROCESSES, Computer Vision and Pattern Recognition, Acoustics and Ultrasonics, Radiology, Nuclear Medicine and imaging, Instrumentation, Materials Science (miscellaneous), General Mathematics, Signal Processing, Biotechnology
title IMAGING OF FLUOROPHORES IN CHROMATOGRAPHIC BEADS, RECONSTRUCTION OF RADIAL DENSITY DISTRIBUTIONS AND CHARACTERISATION OF PROTEIN UPTAKING PROCESSES
title_full IMAGING OF FLUOROPHORES IN CHROMATOGRAPHIC BEADS, RECONSTRUCTION OF RADIAL DENSITY DISTRIBUTIONS AND CHARACTERISATION OF PROTEIN UPTAKING PROCESSES
title_fullStr IMAGING OF FLUOROPHORES IN CHROMATOGRAPHIC BEADS, RECONSTRUCTION OF RADIAL DENSITY DISTRIBUTIONS AND CHARACTERISATION OF PROTEIN UPTAKING PROCESSES
title_full_unstemmed IMAGING OF FLUOROPHORES IN CHROMATOGRAPHIC BEADS, RECONSTRUCTION OF RADIAL DENSITY DISTRIBUTIONS AND CHARACTERISATION OF PROTEIN UPTAKING PROCESSES
title_short IMAGING OF FLUOROPHORES IN CHROMATOGRAPHIC BEADS, RECONSTRUCTION OF RADIAL DENSITY DISTRIBUTIONS AND CHARACTERISATION OF PROTEIN UPTAKING PROCESSES
title_sort imaging of fluorophores in chromatographic beads, reconstruction of radial density distributions and characterisation of protein uptaking processes
title_unstemmed IMAGING OF FLUOROPHORES IN CHROMATOGRAPHIC BEADS, RECONSTRUCTION OF RADIAL DENSITY DISTRIBUTIONS AND CHARACTERISATION OF PROTEIN UPTAKING PROCESSES
topic Computer Vision and Pattern Recognition, Acoustics and Ultrasonics, Radiology, Nuclear Medicine and imaging, Instrumentation, Materials Science (miscellaneous), General Mathematics, Signal Processing, Biotechnology
url http://dx.doi.org/10.5566/ias.v29.p181-189